Curcumin Mediated Gold Nanoparticles and Analysis of its Antioxidant, Anti-inflammatory, Antimicrobial Activity Against Oral Pathogens

ABSTRACT Objective: To green synthesise gold nanoparticles using curcumin and to analyse its antioxidant, anti-inflammatory, and antimicrobial activity among oral pathogens. Material and Methods: Biosynthesised Curcumin Gold nanoparticles (CuAuNP) were evaluated by UV-visible spectrophotometer (UV-Vis), Transmission Electron Microscopy (TEM), and evaluation of antioxidant, anti-inflammatory and antibacterial activity against oral pathogens. Results: Synthesized CuAuNP were characterized using UV-visible spectrophotometry and showed peak absorption at 530nm. CuAuNp showed a 90.3% maximum scavenging ability of DPPH at a concentration of 50 μg/mL. CuAuNP exhibited 79.6 % of the highest anti-inflammatory activity at 50μg/mL than the standard drug diclofenac. TEM image clearly showed uniformly dispersed spherical-shaped gold nanoparticles with a size of about 20 nm. The biosynthesized nanoparticle was tested for its antimicrobial effect, and it showed a potent effect against S. aureus, E. faecalis, and C. albicans at 100µg/ mL. Enterococcus faecalis has a maximum zone of inhibition of 14 mm at 100µg/ mL of CuAuNp. Among gram-positive bacteria, a maximum zone of inhibition of 12 mm at 100µg/ mL was seen in S. aureus compared to S mutans. Candida albicans showed a maximum zone of inhibition of 18 mm at 25 μg/mL of CuAuNp. Conclusion: Curcumin-mediated gold nanoparticles with 20 nm size were effective and had strong antioxidant and anti-inflammatory activity at 50µg/ mL, antimicrobial action inhibiting microbes at 100µg/mL concentration that can be used in treating various Oral mucosal lesions.

Assessment of Nicotine Dependence Using Fagerstrom Test among Patients with Leukoplakia and Oral Submucous Fibrosis

Tobacco consumption has an influence on the oral mucosa and causes oral malignancy in the due time. On assessing nicotine dependence, appropriate counselling can be given to prevent further progression of the habit. Aim of this study was to assess the nicotine dependence levels among patients with potentially malignant disorders such as leukoplakia and Oral Submucous Fibrosis (OSMF) using fagerstrom test. This study included 140 patients from October 2019 to January 2020 in a dental hospital. Variables such as age, gender, tobacco form and lesion were assessed. Descriptive and chi square statistics were performed. Out of 140 patients, higher prevalence with tobacco usage were among 41-50 years (40%), 94.29% were males and 5.71% were females, 16.43% had low dependency, 52.86% had medium dependency and 30.71% had high dependency. Association between   age; nicotine dependency in patients with and without lesion p=0.000(p<0.05), statistically significant. Association between gender and patients with and without lesions p=0.151(p>0.05), statistically not significant. Within the limitations of the present study, males had higher prevalence of tobacco use among 41-50 years. Patients with potentially malignant disorders and smokers without lesion had medium dependence while smokeless tobacco users without lesion had a high dependence